Caister Academic Press

PCR

Author: Aceme Nyika
Easy-to-read handbook for postgraduate students and their supervisors. Explains complex research concepts and methodologies in simple terms.
PCR is the cornerstone of a range of modern scientific disciplines and it is also a key procedure in numerous basic studies involving DNA molecules. All methods for PCR are based on the concept of DNA strand complementarity discovered by James Watson and Francis Crick. PCR became possible with the discovery of DNA polymerases and later their thermostable variants, a variety of isothermal and temperature-cycling amplification techniques have been developed. Little research related to DNA can be performed without the employment of PCR or other DNA amplifi cation procedures.

PCR: The polymerase chain reaction

  • PCR: The polymerase chain reaction A review of PCR applications, procedures, techniques, PCR protocols, PCR troubleshooting, and online resources. PCR is a method for the amplification of DNA that has revolutionised molecular biology and has many and varied applications in other areas of biological and clinical science.

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