The PCR technique is a vital tool in many areas of life sciences. PCR is a method for the amplification of DNA that has revolutionised molecular biology and has many and varied applications in many and diverse areas of biological and clinical science. The PCR techniques in use today are almost exclusively based on real-time PCR, or quantitative PCR (qPCR) technology. Current real-time PCR technology has applications in a huge range of scientific areas. We provide here a review of PCR technology for competent and advanced users. Most of the information provided on these pages is from published books and papers written by leading scientists. The review includes PCR protocols, procedures and techniques, PCR troubleshooting, a description of PCR machines, tips and advice on primer design, PCR applications and much more. Also provided is a full review of PCR theory, both traditional, legacy PCR and real-time quantitative PCR or qPCR.
Edited by: Jim Huggett and Justin O'Grady
"I would highly recommend this book" (Doodys); "useful new insights" (Aus. J. Med. Sci.)
PCR
- Annealing Time and Temperature
- Core-Sampling a PCR Band and Reamplification
- Detection of Amplified DNA
- Diagnostic Real-time PCR Parameters
- Fluorescence and chemiluminescence
- Inadequate dNTPs
- PCR
- Magnesium Concentration
- Multiplex PCR
- Real-time PCR Applications
- PCR: The Basic Theory
- Real-Time PCR Book Reviews
- PCR in Forensic Science
- PCR Papers and articles. Published papers on PCR.
- PCR Primer Design
- PCR Primers
- Polymerase Chain Reaction PCR Protocols
- PCR Technology
- PCR Template DNA
- PCR Theory
- Thermal Cyclers for PCR
- PCR Troubleshooting
- PCR
- Potassium Chloride Concentration
- Primer Concentration
- Principle of PCR
- qPCR Machines and Real-time PCR Platforms
- qPCR Quality Control
- qPCR and Real-Time PCR Web Resources
- PCR Temperature Specifications
- Real-Time PCR Design
- Real-Time PCR for the Detection of Foodborne Pathogens
- An Introduction to Real-Time PCR
- Real-Time PCR Quantification Analysis
- Real-time PCR: The Basic Theory
- Real-Time PCR Technology: Fluorescence Detection
- Real Time PCR
- Taq Concentration
- What is PCR?
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