Caister Academic Press

Real Time PCR

The introduction of novel reagents, chemistries and instrumentation platforms has modified legacy PCR to create a technology, real-time, fluorescence-based quantitative PCR (qPCR). The use of qPCR allows monitoring of the progress of a PCR reaction in real time using fluorescent reporter molecules included in the PCR. In this way parallel and immediate amplification, detection and quantification of PCR products is possible, establishing qPCR firmly as the method of choice for the detection of nucleic acids read more ...
Real-Time PCR: Advanced Technologies and Applications
Edited by: Nick A. Saunders and Martin A. Lee
"an invaluable reference" (Doodys); "wide range of real time PCR technologies" (Food Sci Technol Abs); "I was impressed by this text" Aus J Med Sci

Real Time PCR, Real Time Polymerase Chain Reaction

Real-Time PCR An Introduction to Real-Time PCR

Traditionally, PCR is performed in a tube and when the reaction is complete the products of the reaction (the amplified DNA fragments) are analysed and visualised by gel electrophoresis. However, real-time PCR permits the analysis of the products while the reaction is actually in progress. This is achieved by using various fluorescent dyes which react with the amplified product and can be measured by an instrument. This also facilitates the quantitation of the DNA. Not only can one tell instantly "what" DNA is present in the sample but also "how much". For more detailed information please see Real-Time PCR Theory ...

qPCR machines Real-Time PCR Machines

Real-time PCR primer design Primer Design for Real-Time PCR

Strict guidelines should be observed for optimal and accurate primer design. In any PCR or real-time qPCR procedure it is important to optimise primer design and primer concentration. For more detailed information see PCR Primer Design ...
See also: PCR Troubleshooting and Optimization