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Temperature Specifications of Real-time PCR instruments and qPCR machines

from Julie Logan, Kirstin Edwards and Nick Saunders in Real-Time PCR: Current Technology and Applications
Lab-on-a-Chip Technology
Edited by: Keith E. Herold and Avraham Rasooly
Theoretical and technical information on current LOC technologies, and the design and development of LOC systems, methods and applications.
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Platform

Max. heating/

cooling rate (°C/sec)

Temperature

Accuracy (°C)

Temperature

Uniformity (°C)

ABI 7900HT Fast

3.0/3.0

+/- 0.25

+/- 0.5

ABI 7300

1.1/1.1

+/- 0.25

+/- 0.5

ABI 7500

0.8/1.6

+/- 0.25

+/- 0.5

ABI 7500 Fast

3.5/3.5

+/- 0.25

+/- 0.5

ABI StepOne

2.2/2.2

+/- 0.25

+/- 0.5

Mastercycler ep realplex4

6.0/4.5

+/- 0.2

+/- 0.3

Roche LightCycler

20.0/20.0

+/- 0.4

+/- 0.2

Roche LightCycler 480

4.8 /2.5

+/- 0.2

 

BioRad MiniOpticon

2.5/2.0

+/- 0.2

+/- 0.4

BioRad MyiQ

3.3/2.0

+/- 0.3

+/- 0.4

BioRad Opticon 2

3.0/2.0

+/- 0.4

+/- 0.4

BioRad Chromo4

3.0/2.0

+/- 0.3

+/- 0.4

BioRad iQ5

3.3/2.0

+/- 0.3

+/- 0.4

Stratagene Mx4000

2.2/2.2

+/- 0.25

+/- 0.25

Stratagene Mx3000P

2.5/2.5

+/- 0.25

+/- 0.25

Stratagene Mx3005P

2.5/2.5

+/- 0.25

+/- 0.25

Corbett Rotor-Gene 6000

15.0/20.0

+/- 0.25

+/- 0.01

Cepheid SmartCycler

10.0/2.5

+/- 0.5

+/- 0.5