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Temperature Specifications of Real-time PCR instruments and qPCR machines

from Julie Logan, Kirstin Edwards and Nick Saunders in Real-Time PCR: Current Technology and Applications
Real-Time PCR in Food Science
Edited by: David Rodríguez-Lázaro
An indispensable manual on real-time PCR for scientists in the food industry and for anyone involved in the detection of foodborne pathogens.
"I would recommend this text to anyone" (AJMS); "an excellent, detailed guide" (Emerg. Inf. Dis.) read more ...
Quantitative Real-time PCR in Applied Microbiology
Edited by: Martin Filion
Aimed specifically at microbiologists, this volume describes and explains the most important aspects of current real-time quantitative PCR (qPCR) strategies, instrumentation and software.
"useful book ... filled with valuable information" (Doodys); "an outstanding book" (Fungal Diversity) read more ...

Platform

Max. heating/

cooling rate (°C/sec)

Temperature

Accuracy (°C)

Temperature

Uniformity (°C)

ABI 7900HT Fast

3.0/3.0

+/- 0.25

+/- 0.5

ABI 7300

1.1/1.1

+/- 0.25

+/- 0.5

ABI 7500

0.8/1.6

+/- 0.25

+/- 0.5

ABI 7500 Fast

3.5/3.5

+/- 0.25

+/- 0.5

ABI StepOne

2.2/2.2

+/- 0.25

+/- 0.5

Mastercycler ep realplex4

6.0/4.5

+/- 0.2

+/- 0.3

Roche LightCycler

20.0/20.0

+/- 0.4

+/- 0.2

Roche LightCycler 480

4.8 /2.5

+/- 0.2

 

BioRad MiniOpticon

2.5/2.0

+/- 0.2

+/- 0.4

BioRad MyiQ

3.3/2.0

+/- 0.3

+/- 0.4

BioRad Opticon 2

3.0/2.0

+/- 0.4

+/- 0.4

BioRad Chromo4

3.0/2.0

+/- 0.3

+/- 0.4

BioRad iQ5

3.3/2.0

+/- 0.3

+/- 0.4

Stratagene Mx4000

2.2/2.2

+/- 0.25

+/- 0.25

Stratagene Mx3000P

2.5/2.5

+/- 0.25

+/- 0.25

Stratagene Mx3005P

2.5/2.5

+/- 0.25

+/- 0.25

Corbett Rotor-Gene 6000

15.0/20.0

+/- 0.25

+/- 0.01

Cepheid SmartCycler

10.0/2.5

+/- 0.5

+/- 0.5