PCRPCR is the cornerstone of a range of modern scientific disciplines and it is also a key procedure in numerous basic studies involving DNA molecules. All methods for PCR are based on the concept of DNA strand complementarity discovered by James Watson and Francis Crick. PCR became possible with the discovery of DNA polymerases and later their thermostable variants, a variety of isothermal and temperature-cycling amplification techniques have been developed. Little research related to DNA can be performed without the employment of PCR or other DNA amplifi cation procedures.
Edited by: David Rodríguez-Lázaro"I would recommend this text to anyone" (AJMS); "an excellent, detailed guide" (Emerg. Inf. Dis.) read more ...
An indispensable manual on real-time PCR for scientists in the food industry and for anyone involved in the detection of foodborne pathogens.
Edited by: Martin Filion"useful book ... filled with valuable information" (Doodys); "an outstanding book" (Fungal Diversity) read more ...
Aimed specifically at microbiologists, this volume describes and explains the most important aspects of current real-time quantitative PCR (qPCR) strategies, instrumentation and software.
PCR: The polymerase chain reaction
- PCR: The polymerase chain reaction A review of PCR applications, procedures, techniques, PCR protocols, PCR troubleshooting, and online resources. PCR is a method for the amplification of DNA that has revolutionised molecular biology and has many and varied applications in other areas of biological and clinical science.
- Flow Cytometry in Microbiology
- Probiotics and Prebiotics
- Corynebacterium glutamicum
- Advanced Vaccine Research Methods for the Decade of Vaccines
- Bacteria-Plant Interactions
- Metagenomics of the Microbial Nitrogen Cycle
- Pathogenic Neisseria
- Human Pathogenic Fungi
- Applied RNAi
- Molecular Diagnostics
- Phage Therapy
- Bioinformatics and Data Analysis in Microbiology
- The Cell Biology of Cyanobacteria
- Pathogenic Escherichia coli
- Campylobacter Ecology and Evolution
- Next-generation Sequencing
- Omics in Soil Science
- Applications of Molecular Microbiological Methods