The Theory of Legacy PCR By legacy PCR we mean the original, traditional PCR performed in a tube. When the reaction is complete the products of the reaction (the amplified DNA fragments) are analysed and visualised by gel electrophoresis. For more detailed information see PCR Basic Theory ... The Theory of Real-Time PCR Real-time PCR is achieved by the use of fluorescent dyes that can be measured by a detection system. This also permits the quantitation of the DNA. For more detailed information about real-time PCR please see Real-Time PCR Theory ... The Principle of PCR PCR consists of an exponential amplification of a DNA fragment, and its principle is based on the mechanism of DNA replication in vivo: dsDNA is denatured to ssDNA, duplicated, and this process is repeated along the reaction according to a specific formula. For a more detailed treatment of the principle of PCR please see The Principle of PCR ...
Edited by: Nick A. Saunders and Martin A. Lee"an invaluable reference" (Doodys); "wide range of real time PCR technologies" (Food Sci Technol Abs) read more ...
Provides both the novice and experienced user with an invaluable reference to a wide-range of real-time PCR technologies and applications and supplies detailed technical insights into the underlying principles, methods and practice of real-time PCR.
Edited by: David Rodríguez-Lázaro"I would recommend this text to anyone" (AJMS); "an excellent, detailed guide" (Emerg. Inf. Dis.) read more ...
An indispensable manual on real-time PCR for scientists in the food industry and for anyone involved in the detection of foodborne pathogens.
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