The Theory of Legacy PCR By legacy PCR we mean the original, traditional PCR performed in a tube. When the reaction is complete the products of the reaction (the amplified DNA fragments) are analysed and visualised by gel electrophoresis. For more detailed information see PCR Basic Theory ... The Theory of Real-Time PCR Real-time PCR is achieved by the use of fluorescent dyes that can be measured by a detection system. This also permits the quantitation of the DNA. For more detailed information about real-time PCR please see Real-Time PCR Theory ... The Principle of PCR PCR consists of an exponential amplification of a DNA fragment, and its principle is based on the mechanism of DNA replication in vivo: dsDNA is denatured to ssDNA, duplicated, and this process is repeated along the reaction according to a specific formula. For a more detailed treatment of the principle of PCR please see The Principle of PCR ...
Edited by: Jim Huggett and Justin O'GradyI would highly recommend this book (Doodys) read more ...
The application of molecular technology in clinical diagnosis in two key diagnostic areas: cancer and infectious diseases.
Edited by: Nick A. Saunders and Martin A. Lee"an invaluable reference" (Doodys); "wide range of real time PCR technologies" (Food Sci Technol Abs) read more ...
Provides both the novice and experienced user with an invaluable reference to a wide-range of real-time PCR technologies and applications and supplies detailed technical insights into the underlying principles, methods and practice of real-time PCR.
- Bacterial-Plant Interactions
- Metagenomics of the Microbial Nitrogen Cycle
- Pathogenic Neisseria
- Human Pathogenic Fungi
- Applied RNAi
- Molecular Diagnostics
- Phage Therapy
- Bioinformatics and Data Analysis in Microbiology
- The Cell Biology of Cyanobacteria
- Pathogenic Escherichia coli
- Campylobacter Ecology and Evolution
- Next-generation Sequencing
- Omics in Soil Science
- Applications of Molecular Microbiological Methods
- Genome Analysis
- Bacterial Toxins
- Bacterial Membranes
- Cold-Adapted Microorganisms