PCR

PCR is the cornerstone of a range of modern scientific disciplines and it is also a key procedure in numerous basic studies involving DNA molecules. All methods for PCR are based on the concept of DNA strand complementarity discovered by James Watson and Francis Crick. PCR became possible with the discovery of DNA polymerases and later their thermostable variants, a variety of isothermal and temperature-cycling amplification techniques have been developed. Little research related to DNA can be performed without the employment of PCR or other DNA amplifi cation procedures.

Real-Time PCR in Microbiology: From Diagnosis to Characterization Edited by: Ian M. Mackay
Published: 2007   ISBN: 978-1-904455-18-9
Price: GB £150 or US $300
This invaluable book describes and explains some of the more complex aspects of real-time PCR presenting a background for the novice, a theoretical reference for the experienced user, and useful discussions of future developments. Chapters address the basics of PCR history, oligonucleotide design, target preparation, standardisation, quantification, various applications, and future challenges. The final chapter is presented in the format of a roundtable discussion providing an insightful, topical and interesting discourse with contributions from over 30 authorities and experts on real-time PCR. read more ...
Real-Time PCR: Current Technology and Applications Edited by: Julie Logan, Kirstin Edwards and Nick Saunders
Published: 2009   ISBN: 978-1-904455-39-4
Price: GB £150 or US $310
A comprehensive guide to the most up-to-date real-time PCR technology and applications. The latest PCR platforms, fluorescent chemistries, validation software, data analysis, internal and external controls,clinical diagnostics, biodefense, RNA expression studies, validation of array data, mutation detection, food authenticity and legislation, NASBA, molecular halotyping. read more ...

PCR: The polymerase chain reaction

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