Oligonucleotide microarraysDNA oligonucleotides are spotted onto the array. The main feature of oligonucleotide microarrays is that each gene is normally represented by more than one probe.
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Control, optimize and troubleshoot PCR, reverse transcriptase PCR, real-time PCR and quantitative PCR. An essential book.
Oligonucleotide microarraysIn oligonucleotide microarrays, short DNA oligonucleotides are spotted onto the array. The main feature of oligonucleotide microarray is that each gene is normally represented by more than one probe: the ensemble of probes mapping to different regions of the gene is usually called probe set. Commercial oligonucleotide chips are available (e.g. Affymetrix, Inc. GeneChip system) with an oligonucleotide representation of tens of thousands genes, covering an entire genome; different genomes are currently available (human, mouse, Drosophila and others). For technical reasons, only a single sample can be measured on one of these chips; thus experiments using oligonucleotides microrarrays are often referred as single channel hybridization. This implies that to have data of differential expression between one sample (test sample) and another (reference sample), two separate hybridizations, one for each chip, have to be performed.
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