FluorescenceFluorescence is the emission of electromagnetic radiation, especially of visible light, stimulated in a substance by the absorption of incident radiation and persisting only as long as the stimulating radiation is continued. Fluorescence is important in molecular biology. Fluorescence is used in DNA sequencing. Fluorescence is used in labeling and visualising DNA. Fluorescence is used in DNA chip technology, fluorescence microscopy and green fluorescent protein GFP.
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The most recent advances in NGS instrumentation and data analysis, current NGS platforms, sequencing chemistries, instrument specifications, general workflows and procedures.
FluorescenceFluorescence is important in automated sequencing of DNA. Each of four different chain terminating bases has its own specific fluorescent tag. In fluorescent sequencing the labelled DNA molecules are separated, the fluorescent label is excited by a UV source, and the base is identified by the wavelength of the emitted light. Fluorescence is used in DNA detection. Ethidium bromide has low fluorescence until it binds to DNA. This fluorescence is ussful in visualising the location of DNA fragments in agarose gel electrophoresis. Fluorescence is used in DNA chip technology. Fluorescence is used in microscopy and immunology. FACS (fluorescent-activated cell sorting). Fluorescence can be useful in analysis of the structure and conformations of DNA and proteins. Aequorea victoria, produces a blue fluorescence in the presence of Ca2+ ions. Green Fluorescent Protein (GFP) is an important research tool. The fluorescence of GFP and related proteins are used as reporters in a number of systems. Gene expression can be measured by fluorescence by fusion of a GFP gene to the gene being studied.
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